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验证数据展示

  • WB analysis of mouse heart using 20688-1-AP (same clone as 20688-1-PBS)

    WB analysis of mouse heart using 20688-1-AP (same clone as 20688-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 20688-1-AP (same clone as 20688-1-PBS)

    IHC staining of human kidney using 20688-1-AP (same clone as 20688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAWR antibody included
  • IHC staining of human breast cancer using 20688-1-AP (same clone as 20688-1-PBS)

    IHC staining of human breast cancer using 20688-1-AP (same clone as 20688-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAWR antibody included
  • FC experiment of HeLa using 20688-1-AP (same clone as 20688-1-PBS)

    FC experiment of HeLa using 20688-1-AP (same clone as 20688-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug PAWR Polyclonal antibody (20688-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20688-1-PBS in a different storage buffer formulation.

PAWR Polyclonal antibody, PBS Only

PAWR Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 20688-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PAWR Ready-To-Use IHC Kit. PAWR primary antibody included.

产品信息

20688-1-PBS targets PAWR in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PRKC, apoptosis, WT1, regulator
别名
计算分子量 37 kDa
观测分子量 45 kDa
GenBank蛋白编号NM_002583
基因名称 PAWR
Gene ID (NCBI) 5074
RRIDAB_10733473
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96IZ0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAWR, also named as PAR4, is a pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. It induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. PAWR inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. It down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. PAWR may be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. The antibody is specific to PAWR.