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验证数据展示

  • WB analysis of HepG2 using 55197-1-AP (same clone as 55197-1-PBS)

    WB analysis of HepG2 using 55197-1-AP (same clone as 55197-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55197-1-AP (PAPD5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55197-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 55197-1-AP (same clone as 55197-1-PBS)

    IP experiment of Jurkat using 55197-1-AP (same clone as 55197-1-PBS)

    IP result of anti-PAPD5 (IP:55197-1-AP, 3ug; Detection:55197-1-AP 1:500) with Jurkat cells lysate 1600ug. This data was developed using the same antibody clone with 55197-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PAPD5 Polyclonal antibody, PBS Only

PAPD5 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 55197-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PAPD5 Ready-To-Use IHC Kit. PAPD5 primary antibody included.

产品信息

55197-1-PBS targets PAPD5 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PAP associated domain containing 5
别名
计算分子量 64 kDa
观测分子量 63-80 kDa
GenBank蛋白编号NM_001040285
基因名称 PAPD5
Gene ID (NCBI) 64282
RRIDAB_2881287
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NDF8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAPD5, also named as Non-canonical poly(A) RNA polymerase PAPD5, is a 572 amino acid protein, which contains 1 PAP-associated domain and belongs to the DNA polymerase type-B-like family. PAPD5 is predominantly expressed in the cytoplasm. PAPD5 as catalytic subunit of a TRAMP-like complex that has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. PAPD5 plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. PAPD5 also may play a role in sister chromatid cohesion. The calculated molecular weight of PAPD5 is a 63 kDa, but the post-modified protein is about 70-80 kDa. PAPD5 exists some isoform with the molecular weight about 50 kDa and 70-80 kDa.