P21 Polyclonal antibody
P21 Polyclonal Antibody for IF/ICC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC,ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IF detected in | myotome of a somite at E11.5, SH-SY5Y cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10240-1-AP targets P21 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC,ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | P21 fusion protein Ag0308 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Synonyms | |
| Calculated Molecular Weight | 21 kDa |
| GenBank Accession Number | BC000312 |
| Gene Symbol | CDKN1A |
| Gene ID (NCBI) | 1026 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P38936 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.
发表文章
| Species | Application | Title |
|---|---|---|
PLoS One FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short. |
