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验证数据展示

  • WB analysis of mouse liver using 25137-1-AP (same clone as 25137-1-PBS)

    WB analysis of mouse liver using 25137-1-AP (same clone as 25137-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25137-1-AP (ONECUT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    IHC staining of human liver cancer using 25137-1-AP (same clone as 25137-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25137-1-AP (ONECUT1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25137-1-PBS in a different storage buffer formulation.

ONECUT1 Polyclonal antibody, PBS Only

ONECUT1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25137-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25137-1-PBS targets ONECUT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18250

Product name: Recombinant human ONECUT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 167-292 aa of BC140830

Sequence: PYHKDVAGMGQSLSPLSSSGLGSIHNSQQGLPHYAHPGAAMPTDKMLTPNGFEAHHPAMLGRHGEQHLTPTSAGMVPINGLPPHHPHAHLNAQGHGQLLGTAREPNPSVTGAQVSNGSNSGQMEEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 one cut homeobox 1
别名
计算分子量 465 aa, 51 kDa
观测分子量 51-55 kDa
GenBank蛋白编号BC140830
基因名称 ONECUT1
Gene ID (NCBI) 3175
RRIDAB_2879918
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBC0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ONECUT1, also named as Hepatocyte nuclear factor 6 (HNF6), is a 465 amino acid protein, which contains one CUT DNA-binding domain and one homeobox DNA-binding domain. ONECUT1 localizes in the nucleus and belongs to the CUT homeobox family. ONECUT1 as a transcription activator is involved in the some liver genes transcription. ONECUT1 is highly expressed in liver and lower in testis, skin.

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