验证数据展示
经过测试的应用
| Positive WB detected in | HuH-7 cells, U-251 cells, mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31165-1-AP targets OLFML1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | OLFML1 fusion protein Ag35125 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | olfactomedin-like 1 |
| 别名 | UNQ564, olfactomedin like 1 |
| 计算分子量 | 402 aa, 46 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC092488 |
| 基因名称 | OLFML1 |
| Gene ID (NCBI) | 283298 |
| RRID | AB_3669879 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6UWY5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OLFML1 (Olfactomedin-like protein 1) is a novel member of human OLF protein family with an olfectamine domain (143-397 aa) in its C-terminus. OLFML1 exhibits the promotion of cell proliferation, along with the increase of percentage of S phase cells. OLFML1 is considered a secreted glycoprotein that enhances cell cycle progression in human cancer cell lines in vitro (PMID: 30266405). OLFML1 contains three potential N-glycosylation sites (residues 66, 138 and 183) and could be modified by the N-glycosylation (PMID: 18708057).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OLFML1 antibody 31165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |