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PE-conjugated NeutraKine® TGF beta 1 Monoclonal antibody

NeutraKine® TGF beta 1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

PE Fluorescent Dye

CloneNo.

1E3E9

Cat No : PE-69012

Print datasheet

Synonyms

CED, DPD1, LAP, TGF ?1, TGF beta 1, TGFB, TGFB1, TGFbeta

验证数据展示

  • FC experiment of HEK-293 using PE-69012

    FC experiment of HEK-293 using PE-69012

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug PE Anti-Human NeutraKine® TGF beta 1 (PE-69012, Clone:1E3E9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TGF beta 1 Ready-To-Use IHC Kit. TGF beta 1 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-69012 targets NeutraKine® TGF beta 1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen NeutraKine® TGF beta 1 fusion protein HZ-1011 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transforming growth factor, beta 1
Synonyms CED, DPD1, LAP, TGF ?1, TGF beta 1, TGFB, TGFB1, TGFbeta
Gene Symbol TGFB1
Gene ID (NCBI) 7040
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody can be used to neutralize the bioactivity of TGF beta 1.

实验方案

Product Specific Protocols
FC protocol for PE NeutraKine® TGF beta 1 antibody PE-69012Download protocol
Standard Protocols
Click here to view our Standard Protocols