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验证数据展示

  • IF Staining of Human dorsal cerebral organoid using 25265-1-AP (same clone as 25265-1-PBS)

    IF Staining of Human dorsal cerebral organoid using 25265-1-AP (same clone as 25265-1-PBS)

    Human dorsal cerebral organoid at 12 weeks. 4% PFA fixed. Antibody dilution was 1:500 for Syne-2 (25265-1-AP; white), DAPI 1:1000. Image created by Wimmer Ryszard from the Institut Curie in Paris, France This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 25265-1-AP (same clone as 25265-1-PBS)

    WB analysis of HEK-293 using 25265-1-AP (same clone as 25265-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25265-1-AP (SYNE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 25265-1-AP (same clone as 25265-1-PBS)

    IF Staining of mouse brain using 25265-1-AP (same clone as 25265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 25265-1-AP (same clone as 25265-1-PBS)

    IF Staining of mouse brain using 25265-1-AP (same clone as 25265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 25265-1-AP (same clone as 25265-1-PBS)

    IF Staining of rat brain using 25265-1-AP (same clone as 25265-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

Nesprin 2 Polyclonal antibody, PBS Only

Nesprin 2 Polyclonal Antibody for WB, IF, Indirect ELISA
Cat No. 25265-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25265-1-PBS targets Nesprin 2 in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spectrin repeat containing, nuclear envelope 2
别名
计算分子量 799 kDa
观测分子量 50-60 kDa
GenBank蛋白编号NM_015180
基因名称 Nesprin 2
Gene ID (NCBI) 23224
RRIDAB_2879995
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXH0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.