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验证数据展示

  • WB analysis of mouse small intestine using 27190-1-AP (same clone as 27190-1-PBS)

    WB analysis of mouse small intestine using 27190-1-AP (same clone as 27190-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27190-1-AP (NHE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH9). Arrows show brush border. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    IHC staining of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27190-1-AP (NHE3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    IP experiment of mouse kidney using 27190-1-AP (same clone as 27190-1-PBS)

    IP result of anti-NHE3 (IP:27190-1-AP, 4ug; Detection:27190-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 27190-1-PBS in a different storage buffer formulation.

NHE3 Polyclonal antibody, PBS Only

NHE3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27190-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

SLC9A3, NHE-3

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

27190-1-PBS targets NHE3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NHE3 fusion protein Ag25637 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 9 (sodium/hydrogen exchanger), member 3
别名 SLC9A3, NHE-3
计算分子量 834 aa, 93 kDa
观测分子量 80-100 kDa
GenBank蛋白编号BC101669
基因名称 NHE3
Gene ID (NCBI) 6550
RRIDAB_2880793
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48764
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC9A3, also known as NHE3, belongs to the sodium hydrogen exchange protein family (NHE) and is mainly responsible for regulating the concentration of sodium and hydrogen ions inside and outside the cell. SLC9A3 is a Major apical Na+/H+ exchanger in kidney and intestine, playing an important role in renal and intestine Na+ absorption and blood pressure regulation (PMID:24622516, 2635877). Mutations in SLC9A3 cause Congenital Sodium Diarrhea (PMID: 26358773, 31276831).