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Species Reactivity
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Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 10233-1-AP (same clone as 10233-1-PBS)

    WB analysis using 10233-1-AP (same clone as 10233-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

    IF Staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using NDE1 antibody (10233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

    IF Staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using NDE1 antibody (10233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

  • WB analysis of A2780 using 10233-1-AP (same clone as 10233-1-PBS)

    WB analysis of A2780 using 10233-1-AP (same clone as 10233-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 10233-1-AP (NDE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10233-1-AP (same clone as 10233-1-PBS)

    IP experiment of HEK-293 using 10233-1-AP (same clone as 10233-1-PBS)

    IP result of anti-NDE1 (IP:10233-1-AP, 3ug; Detection:10233-1-AP 1:1500) with HEK-293 cells lysate 4500ug. This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

    IHC staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

    IHC staining of human breast cancer using 10233-1-AP (same clone as 10233-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10233-1-AP (NDE1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 10233-1-AP (same clone as 10233-1-PBS)

    IF Staining of MCF-7 using 10233-1-AP (same clone as 10233-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NDE1 antibody (10233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10233-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NDE1 Polyclonal antibody, PBS Only

NDE1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 10233-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

10233-1-PBS targets NDE1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NDE1 fusion protein Ag0336 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nudE nuclear distribution gene E homolog 1 (A. nidulans)
别名
计算分子量 335 aa, 38 kDa
观测分子量 40 kDa
GenBank蛋白编号BC001421
基因名称 NDE1
Gene ID (NCBI) 54820
RRIDAB_2149877
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXR1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.