Speedy™ Mouse/Rat IGF-1 One-Step ELISA Kit
Sensitivity
1.5 pg/mL
Range
7.8-500 pg/mL
Reactivity
Mouse/Rat
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse/Rat IGF-1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma, Rat serum, Rat plasma, Cell culture supernatant, Tissue lysate, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24482 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 95% | 93%-97% |
| Rat serum | 94% | 92%-97% |
| Cell culture supernatant | 91% | 88%-94% |
| Tissue lysate | 95% | 91%-98% |
| Tissue homogenates | 97% | 91%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 249.1 | 12.5 | 5.0 |
| 2 | 8 | 62.8 | 2.2 | 3.5 |
| 3 | 8 | 30.1 | 0.6 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 245.2 | 14.5 | 5.9 |
| 2 | 16 | 60.7 | 2.9 | 4.8 |
| 3 | 16 | 29.7 | 1.0 | 3.4 |
Background Information
Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone, is the principal mediator of biochemical effects of growth hormone (GH) and important in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas. Mice lacking IGF1 exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Igf1, IGF-I, Insulin-like growth factor I, Somatomedin |
