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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30145-1-AP (same clone as 30145-1-PBS)

    WB analysis using 30145-1-AP (same clone as 30145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (MLKL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30145-1-PBS in a different storage buffer formulation.

  • WB analysis using 30145-1-AP (same clone as 30145-1-PBS)

    WB analysis using 30145-1-AP (same clone as 30145-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30145-1-AP (MLKL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30145-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 30145-1-AP (same clone as 30145-1-PBS)

    IF Staining of NIH/3T3 using 30145-1-AP (same clone as 30145-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MLKL antibody (30145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30145-1-PBS in a different storage buffer formulation.

Mouse MLKL Polyclonal antibody, PBS Only

MLKL Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30145-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

mixed lineage kinase domain like

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

30145-1-PBS targets MLKL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 mouse, rat
免疫原 MLKL fusion protein Ag32514 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mixed lineage kinase domain-like
别名 mixed lineage kinase domain like
计算分子量54kd
观测分子量 54 kDa
GenBank蛋白编号NM_001310613
基因名称 Mlkl
Gene ID (NCBI) 74568
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9D2Y4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mixed lineage kinase domain-like pseudokinase (Mlkl) belongs to the protein kinase superfamily and has two MWs of 54 and 30 kDa. Mlkl plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in the necroptosis pathway. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.