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验证数据展示

  • WB analysis using 16963-1-AP (same clone as 16963-1-PBS)

    WB analysis using 16963-1-AP (same clone as 16963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16963-1-AP (MYL6B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16963-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 16963-1-AP (same clone as 16963-1-PBS)

    IHC staining of mouse skeletal muscle using 16963-1-AP (same clone as 16963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16963-1-AP (MYL6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16963-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 16963-1-AP (same clone as 16963-1-PBS)

    IHC staining of mouse skeletal muscle using 16963-1-AP (same clone as 16963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16963-1-AP (MYL6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16963-1-PBS in a different storage buffer formulation.

MYL6B Polyclonal antibody, PBS Only

MYL6B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16963-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16963-1-PBS targets MYL6B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10683

Product name: Recombinant human MYL6B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-208 aa of BC012425

Sequence: MPPKKDVPVKKPAGPSISKPAAKPAAAGAPPAKTKAEPAVPQAPQKTQEPPVDLSKVVIEFNKDQLEEFKEAFELFDRVGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDELKSRRVDFETFLPMLQAVAKNRGQGTYEDYLEGFRVFDKEGNGKVMGAELRHVLTTLGEKMTEEEVETVLAGHEDSNGCINYEAFLKHILSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myosin, light chain 6B, alkali, smooth muscle and non-muscle
别名
计算分子量 208 aa, 23 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC012425
基因名称 MYL6B
Gene ID (NCBI) 140465
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14649
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYL6B, also known as MLC1SA, is primarily found in a hexamer consisting of four light chains and two heavy chains. MYL6B is an essential light chain for non-muscle Myosin II (NMII) that is involved in the control of cell adhesion, cell migration and tissue architecture, cargo transport, and endocytosis (PMID: 29439719, 33817240).

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