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验证数据展示

  • WB analysis using 13397-1-AP (same clone as 13397-1-PBS)

    WB analysis using 13397-1-AP (same clone as 13397-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 13397-1-AP (MIP-3 Beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    IF Staining of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using MIP-3 Beta antibody (13397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    FC experiment of A549 using 13397-1-AP (same clone as 13397-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human MIP-3 Beta (13397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13397-1-PBS in a different storage buffer formulation.

CCL19/MIP-3 beta Polyclonal antibody, PBS Only

CCL19/MIP-3 beta Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 13397-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

CCL19, MIP-3 Beta, MIP-3-beta, Beta chemokine exodus 3, Beta-chemokine exodus-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13397-1-PBS targets CCL19/MIP-3 beta in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4200

Product name: Recombinant human MIP­3β protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-98 aa of BC027968

Sequence: MALLLALSLLVLWTSPAPTLSGTNDAEDCCLSVTQKPIPGYIVRNFHYLLIKDGCRVPAVVFTTLRGRQLCAPPDQPWVERIIQRLQRTSAKMKRRSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) ligand 19
别名 CCL19, MIP-3 Beta, MIP-3-beta, Beta chemokine exodus 3, Beta-chemokine exodus-3
计算分子量 98 aa, 12 kDa
观测分子量11 kDa
GenBank蛋白编号BC027968
基因名称 CCL19/MIP-3 beta
Gene ID (NCBI) 6363
RRIDAB_2071529
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99731
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCL19, also known as MIP-3β, is one of several CC cytokine genes clustered on the p-arm of chromosome 9. CCL19 may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7.

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