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验证数据展示

  • WB analysis using 16983-1-AP (same clone as 16983-1-PBS)

    WB analysis using 16983-1-AP (same clone as 16983-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16983-1-AP (METTL2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 16983-1-AP (same clone as 16983-1-PBS)

    IF Staining of MCF-7 using 16983-1-AP (same clone as 16983-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (16983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 16983-1-AP (same clone as 16983-1-PBS)

    IHC staining of mouse colon using 16983-1-AP (same clone as 16983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16983-1-AP (METTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

METTL2 Polyclonal antibody, PBS Only

METTL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16983-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16983-1-PBS targets METTL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10410

Product name: Recombinant human METTL2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC107586

Sequence: MPVDYEINAHKYWNDFYKIHENGFFKDRHWLFTEFPELAPSQNQNHLKDWFLENKSEVCECRNNEDGPGLIMEEQHKCSSKSLEHKTQTPPVEENVTQKISDLEICADEFPGSSATYRILEVGCGVGNTVFPILQTNNDPGLFVYCCDFSSTAIELVQTNSEYDPSRCFAFVHDLCDEEKSYPVPKGSLDIIILIFVLSAIVPDKMQKAINRLSRLLKPGGMVLLRDYGRYDMAQLRFKKGQCLSGNFYVRGDGTRVYFFTQEELDTLFTTAGLEKVQNLVDRRLQVNRGKQLTMYRVWIQCKYCKPLLSSTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methyltransferase like 2B
别名
计算分子量 313 aa, 36 kDa
观测分子量36 kDa
GenBank蛋白编号BC107586
基因名称 METTL2B
Gene ID (NCBI) 55798
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P1Q9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.

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