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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 28702-1-AP (same clone as 28702-1-PBS)

    WB analysis using 28702-1-AP (same clone as 28702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28702-1-AP (METAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

  • WB analysis using 28702-1-AP (same clone as 28702-1-PBS)

    WB analysis using 28702-1-AP (same clone as 28702-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28702-1-AP (METAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 28702-1-AP (same clone as 28702-1-PBS)

    IHC staining of human ovary cancer using 28702-1-AP (same clone as 28702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28702-1-AP (METAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 28702-1-AP (same clone as 28702-1-PBS)

    IF Staining of HEK-293 using 28702-1-AP (same clone as 28702-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using METAP1 antibody (28702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28702-1-PBS in a different storage buffer formulation.

METAP1 Polyclonal antibody, PBS Only

METAP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28702-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Peptidase M 1, MetAP 1, MAP 1, KIAA0094

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

28702-1-PBS targets METAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 METAP1 fusion protein Ag29828 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 methionyl aminopeptidase 1
别名 Peptidase M 1, MetAP 1, MAP 1, KIAA0094
计算分子量 43 kDa
观测分子量40-43 kDa
GenBank蛋白编号NM_015143
基因名称 METAP1
Gene ID (NCBI) 23173
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53582
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

METAP1 (methionyl aminopeptidase 1), also known as MAP1A or MetAP1A, is expected to be located in cytosol and widely expressed in esophagus and testis. The calculated molecular weight of METAP1 is 43 kDa. METAP1 is expected to have aminopeptidase activity and metallo-exopeptidase activity. It is also involved in platelet aggregation. At the same time, HsMetAP1 participates in co-translation to remove N- terminal methionine from newborn protein. Studies have shown that when HsMetAP1 is knocked down by gene-specific siRNA, cells show slow progress in G(2)/M phase, which is similar to cells treated with MetAP1 inhibitors. Importantly, MetAP1 inhibitors can induce apoptosis of leukemia cell lines, which may be the result of their interference with G(2)/M checkpoint. Therefore, it is necessary for normal cell cycle, especially in G(2)/M phase of cell cycle, and it can be used as a promising target for discovering and developing new anticancer drugs (PMID: 17114291).