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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 25615-1-AP (same clone as 25615-1-PBS)

    WB analysis using 25615-1-AP (same clone as 25615-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25615-1-AP (MBOAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25615-1-PBS in a different storage buffer formulation.

MBOAT1 Polyclonal antibody, PBS Only

MBOAT1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 25615-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MBOAT1 Ready-To-Use IHC Kit. MBOAT1 primary antibody included.

产品信息

25615-1-PBS targets MBOAT1 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 MBOAT1 fusion protein Ag22470 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane bound O-acyltransferase domain containing 1
别名
计算分子量 495 aa, 57 kDa
观测分子量45-57 kDa
GenBank蛋白编号BC150652
基因名称 MBOAT1
Gene ID (NCBI) 154141
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZNC8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MBOAT1 (membrane bound O-acyltransferase domain containing 1), also known as LPLAT or LPSAT. It is expected to be located in membrane and widely expressed in colon, small intestine. MBOAT1 belongs to the membrane-bound O- acetyltransferase superfamily. Its encoded transmembrane protein is an enzyme that transfers organic compounds, preferably oleoyl-CoA, to the hydroxyl group of protein target in the membrane. The translocation that destroys the gene may be related to short finger toe syndrome. At the same time, it may also play a role in neurite growth during neuronal differentiation (PMID: 18772128). Studies have determined that phospholipid modifying enzymes MBOAT1 and MBOAT2 are iron sag inhibitors. MBOAT1/2 can inhibit iron sag by reshaping the phospholipid profile of cells, and their iron sag monitoring function is independent of GPX4 or FSP1. MBOAT1 and MBOAT2 are up-regulated by sex hormone receptors, namely estrogen receptor (ER) and androgen receptor (AR), respectively (PMID: 37267948). The calculated molecular weight of MBOAT1 is 57 kDa. This antibody can recognize the 40 kDa isoform of MBOAT1.