IHCeasy MBOAT1 Ready-To-Use IHC Kit
MBOAT1 Ready-to-use reagent kit for IHC.
Reactivity
Human
Sample Type
FFPE tissue
Cat No : KHC2602
Synonyms
MBOAT1,1-acylglycerophosphocholine O-acyltransfera
Validation Data Gallery
Product Information
KHC2602 is a ready-to-use IHC kit for staining of MBOAT1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy MBOAT1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
MBOAT1 (membrane bound O-acyltransferase domain containing 1), also known as LPLAT or LPSAT. It is expected to be located in membrane and widely expressed in colon, small intestine. MBOAT1 belongs to the membrane-bound O- acetyltransferase superfamily. Its encoded transmembrane protein is an enzyme that transfers organic compounds, preferably oleoyl-CoA, to the hydroxyl group of protein target in the membrane. The translocation that destroys the gene may be related to short finger toe syndrome. At the same time, it may also play a role in neurite growth during neuronal differentiation. Studies have determined that phospholipid modifying enzymes MBOAT1 and MBOAT2 are iron sag inhibitors. MBOAT1/2 can inhibit iron sag by reshaping the phospholipid profile of cells, and their iron sag monitoring function is independent of GPX4 or FSP1. MBOAT1 and MBOAT2 are up-regulated by sex hormone receptors, namely estrogen receptor (ER) and androgen receptor (AR), respectively.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | MBOAT1,1-acylglycerophosphocholine O-acyltransfera |