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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 30716-1-AP (same clone as 30716-1-PBS)

    WB analysis using 30716-1-AP (same clone as 30716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30716-1-AP (MAP4K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30716-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 30716-1-AP (same clone as 30716-1-PBS)

    IHC staining of mouse skeletal muscle using 30716-1-AP (same clone as 30716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30716-1-AP (MAP4K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30716-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 30716-1-AP (same clone as 30716-1-PBS)

    IHC staining of mouse skeletal muscle using 30716-1-AP (same clone as 30716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 30716-1-AP (MAP4K3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30716-1-PBS in a different storage buffer formulation.

  • IF Staining of U-87 MG using 30716-1-AP (same clone as 30716-1-PBS)

    IF Staining of U-87 MG using 30716-1-AP (same clone as 30716-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using MAP4K3 antibody (30716-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30716-1-PBS in a different storage buffer formulation.

MAP4K3 Polyclonal antibody, PBS Only

MAP4K3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30716-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MEKKK 3, MEK kinase kinase 3, MAPKKKK3, GLK, Germinal center kinase-related protein kinase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30716-1-PBS targets MAP4K3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MAP4K3 fusion protein Ag33512 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase kinase 3
别名 MEKKK 3, MEK kinase kinase 3, MAPKKKK3, GLK, Germinal center kinase-related protein kinase
计算分子量 101 kDa
观测分子量 99-101 kDa
GenBank蛋白编号BC071579
基因名称 MAP4K3
Gene ID (NCBI) 8491
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVH8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP4K3(Mitogen-activated protein kinase kinase kinase kinase 3) has kinase activity and that it activates JNK but not MAPK1, MAPK3 or MAPK14 through the activation of MAP3K1 and MAP2K4. Endogenous MAP4K3 kinase activity could be stimulated by exposure to ultraviolet radiation or to tumor necrosis factor.