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验证数据展示

  • WB analysis of HeLa using 18278-1-AP (same clone as 18278-1-PBS)

    WB analysis of HeLa using 18278-1-AP (same clone as 18278-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18278-1-AP (LMX1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18278-1-PBS in a different storage buffer formulation.

  • IF Staining of embryonic kidney tissue using 18278-1-AP (same clone as 18278-1-PBS)

    IF Staining of embryonic kidney tissue using 18278-1-AP (same clone as 18278-1-PBS)

    IF result of anti-LMX1B (18278-1-AP ,1:900) with embryonic kidney by Dr. Joo-Seop Park. Red: Podocytes, anti-rabbir Alexa555; Blue: Hoechst. This data was developed using the same antibody clone with 18278-1-PBS in a different storage buffer formulation.

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  • KD/KO验证

LMX1B Polyclonal antibody, PBS Only

LMX1B Polyclonal Antibody for WB, IF, Indirect ELISA
Cat No. 18278-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF, Indirect ELISA

别名

LIM homeobox transcription factor 1-beta, LMX 1.2, LMX1, LMX1.2, LMX-1.2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

18278-1-PBS targets LMX1B in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13096

Product name: Recombinant human LMX1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-372 aa of BC112120

Sequence: MLDGIKMEEHALRPGPATLGVLLGSDCPHPAVCEGCQRPISDRFLMRVNESSWHEECLQCAACQQALTTSCYFRDRKLYCKQDYQQLFAAKCSGCMEKIAPTEFVMRALECVYHLGCFCCCVCERQLRKGDEFVLKEGQLLCKGDYEKEKDLLSSVSPDESDSVKSEDEDGDMKPAKGQGSQSKGSGDDGKDPRRPKRPRTILTTQQRRAFKASFEVSSKPCRKVRETLAAETGLSVRVVQVWFQNQRAKMKKLARRHQQQQEQQNSQRLGQEVLSSRMEGMMASYTPLAPPQQQIVAMEQSPYGSSDPFQQGLTPPQMPGNDSIFHDIDSDTSLTSLSDCFLGSSDVGSLQARVGNPIDRLYSMQSSYFAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM homeobox transcription factor 1, beta
别名 LIM homeobox transcription factor 1-beta, LMX 1.2, LMX1, LMX1.2, LMX-1.2
计算分子量 372 aa, 42 kDa
观测分子量 42-45 kDa
GenBank蛋白编号BC112120
基因名称 LMX1B
Gene ID (NCBI) 4010
RRIDAB_11232226
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60663
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LMX1B, LIM homeodomain factor 1b, functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Lmx1b is a continuously expressed, terminal selector-type factor in 5-HT neurons. It is required for maintenance of central serotonergic neurons (PMID: 17151281, PMID: 14602809). It is expressed in brain, testis, skeletal muscle, and pancreatic islets (PMID: 35417751, PMID: 18512225).

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