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验证数据展示

  • WB analysis of HeLa using 20772-1-AP (same clone as 20772-1-PBS)

    WB analysis of HeLa using 20772-1-AP (same clone as 20772-1-PBS)

    WB result of LIMS1 antibody (20772-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected HeLa cells. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • WB analysis using 20772-1-AP (same clone as 20772-1-PBS)

    WB analysis using 20772-1-AP (same clone as 20772-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20772-1-AP (LIMS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 20772-1-AP (same clone as 20772-1-PBS)

    IP experiment of K-562 using 20772-1-AP (same clone as 20772-1-PBS)

    IP result of anti-LIMS1 (IP:20772-1-AP, 4ug; Detection:20772-1-AP 1:800) with K-562 cells lysate 1640 ug. This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 20772-1-AP (same clone as 20772-1-PBS)

    IHC staining of human colon cancer using 20772-1-AP (same clone as 20772-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20772-1-AP (LIMS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20772-1-AP (same clone as 20772-1-PBS)

    IF Staining of HeLa using 20772-1-AP (same clone as 20772-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LIMS1 antibody (20772-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20772-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

LIMS1 Polyclonal antibody, PBS Only

LIMS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20772-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH, PINCH 1, PINCH1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

20772-1-PBS targets LIMS1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14764

Product name: Recombinant human LIMS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-325 aa of BC005341

Sequence: MANALASATCERCKGGFAPAEKIVNSNGELYHEQCFVCAQCFQQFPEGLFYEFEGRKYCEHDFQMLFAPCCHQCGEFTIGRVIKAMNNSWHPECFRCDLCQEVLADIGFVKNAGRHLCRPCHNREKARGLGKYICQKCHAIIDEQPLIFKNDPYHPDHFNCANCGKELTADARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNAMGKQWHVEHFVCAKCEKPFLGHRHYERKGLAYCETHYNQLFGDVCFHCNRVIEGGVVSALNKAWCVNCFACSTCNTKLTLKNKFVEFDMKPVCKKCYEKFPLELKKRLKKLAETLGRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM and senescent cell antigen-like domains 1
别名 LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH, PINCH 1, PINCH1
计算分子量 325 aa, 37 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC005341
基因名称 LIMS1
Gene ID (NCBI) 3987
RRIDAB_10695765
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48059
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LIMS1, also known as PINCH1, is an adaptor protein that contains five LIM domains, or double zinc fingers(PMID: 7517666). PINCH-1 is a widely expressed focal adhesion protein that forms a ternary complex with integrin-linked kinase (ILK) and CH-ILKBP/actopaxin/alpha-parvin (PMID: 14551191). LIMS1 is involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.

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