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验证数据展示

  • WB analysis using 32885-1-AP

    WB analysis using 32885-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32885-1-AP (L1CAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 32885-1-AP

    IHC staining of mouse cerebellum using 32885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 32885-1-AP

    IHC staining of mouse cerebellum using 32885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

L1CAM/CD171 Polyclonal antibody

L1CAM/CD171 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32885-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

L1cam, N-CAM-L1, CAML1, CD171, N CAM L1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32885-1-AP targets L1CAM/CD171 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 L1CAM/CD171 fusion protein Eg3022 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 L1 cell adhesion molecule
别名 L1cam, N-CAM-L1, CAML1, CD171, N CAM L1
计算分子量141 kDa
观测分子量200-220 kDa, 140 kDa
GenBank蛋白编号N/A
基因名称 L1cam
Gene ID (NCBI) 16728
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP11627
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733).

实验方案

Product Specific Protocols
WB protocol for L1CAM/CD171 antibody 32885-1-APDownload protocol
IHC protocol for L1CAM/CD171 antibody 32885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols