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验证数据展示

  • IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)

    IHC staining of human lymphoma using 18131-1-AP (same clone as 18131-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 18131-1-AP (FERMT3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • WB analysis using 18131-1-AP (same clone as 18131-1-PBS)

    WB analysis using 18131-1-AP (same clone as 18131-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18131-1-AP (Kindlin 3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 18131-1-AP (same clone as 18131-1-PBS)

    IF Staining of THP-1 using 18131-1-AP (same clone as 18131-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Kindlin 3 antibody (18131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 18131-1-AP (same clone as 18131-1-PBS)

    IF Staining of Raji using 18131-1-AP (same clone as 18131-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Kindlin 3 antibody (18131-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18131-1-PBS in a different storage buffer formulation.

Kindlin 3 Polyclonal antibody, PBS Only

Kindlin 3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18131-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FERMT3, KIND3, Kindlin-3, MIG 2, MIG2 like protein

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

18131-1-PBS targets Kindlin 3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12713

Product name: Recombinant human FERMT3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 321-663 aa of BC004347

Sequence: DDLDVALSNLEVKLEGSAPTDVLDSLTTIPELKDHLRIFRPRKLTLKGYRQHWVVFKETTLSYYKSQDEAPGDPIQQLNLKGCEVVPDVNVSGQKFCIKLLVPSPEGMSEIYLRCQDEQQYARWMAGCRLASKGRTMADSSYTSEVQAILAFLSLQRTGSGGPGNHPHGPDASAEGLNPYGLVAPRFQRKFKAKQLTPRILEAHQNVAQLSLAEAQLRFIQAWQSLPDFGISYVMVRFKGSRKDEILGIANNRLIRIDLAVGDVVKTWRFSNMRQWNVNWDIRQVAIEFDEHINVAFSCVSASCRIVHEYIGGYIFLSTRERARGEELDEDLFLQLTGGHEAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fermitin family homolog 3 (Drosophila)
别名 FERMT3, KIND3, Kindlin-3, MIG 2, MIG2 like protein
计算分子量 76 kDa
观测分子量 72-76 kDa
GenBank蛋白编号BC004347
基因名称 Kindlin 3/FERMT3
Gene ID (NCBI) 83706
RRIDAB_2103056
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UX7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kindlin-3 (FERMT-3) is known to be central in hemostasis and thrombosis control and its deficiency disrupts platelet aggregation and causes Leukocyte Adhesion Deficiency disease. Point mutations in the kindlin-3 gene have been identified in humans with a rare inherited Integrin Activation efficiency Disease (IADD), also designated as Leukocyte Adhesion Deficiency syndrome (LAD-III or LADI variant). Kindlin-3 expression is restricted to cells of hematopoietic origin.

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