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验证数据展示

  • WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

    WB analysis using 11342-1-AP (same clone as 11342-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11342-1-AP (KRT81 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11342-1-AP (same clone as 11342-1-PBS)

    IHC staining of human colon cancer using 11342-1-AP (same clone as 11342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11342-1-AP (KRT81 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KRT81 antibody included
  • IP experiment of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

    IP experiment of HEK-293 using 11342-1-AP (same clone as 11342-1-PBS)

    IP result of anti-KRT81 (IP:11342-1-AP, 4ug; Detection:11342-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11342-1-AP (same clone as 11342-1-PBS)

    IF Staining of HeLa using 11342-1-AP (same clone as 11342-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRT81 antibody (11342-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11342-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Cytokeratin 81 Polyclonal antibody, PBS Only

Cytokeratin 81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11342-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

KRT81, Cytokeratin,Keratin, ghHb1, keratin 81, Keratin-81

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KRT81 Ready-To-Use IHC Kit. KRT81 primary antibody included.

产品信息

11342-1-PBS targets Cytokeratin 81 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1890

Product name: Recombinant human KRT81 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-202 aa of BC021241

Sequence: MKATVIRHGETLRRTKEEINELNRMIQRLTAEVENAKCQNSKLEAAVAQSEQQGEAALSDARCKLAELEGALQKAKQDMACLIREYQEVMNSKLGLDIEIATYRRLLEGEEQRLCEGIGAVNVCVSSSRGGVVCGDLCVSGSRPVTGSVCSAPCNGNVAVSTGLCAPCGQLNTTCGGGSCGVGSCGISSLGVGSCGSSCRKC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 81
别名 KRT81, Cytokeratin,Keratin, ghHb1, keratin 81, Keratin-81
计算分子量 55 kDa
观测分子量 54 kDa
GenBank蛋白编号BC021241
基因名称 KRT81
Gene ID (NCBI) 3887
RRIDAB_2132771
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14533
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT81 is a type II keratin.

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