验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31197-1-AP targets Keap1 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 | Keap1 fusion protein Ag35035 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kelch-like ECH-associated protein 1 |
| 别名 | INRF2, mKIAA0132 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 60-64 kDa |
| GenBank蛋白编号 | NM_001110305 |
| 基因名称 | Keap1 |
| Gene ID (NCBI) | 50868 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Z2X8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KEAP1 (Kelch-like ECH-associated protein 1) is a critical regulatory protein that plays a pivotal role in the cellular response to oxidative stress. It is best known for its function as a negative regulator of the Nrf2 (NF-E2-related factor 2) pathway, which is essential for maintaining cellular redox homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Keap1 antibody 31197-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |