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CoraLite®594-conjugated KMO Monoclonal antibody

KMO Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D2F2

Cat No : CL594-60029

Print datasheet

Synonyms

dJ317G22.1, KMO, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase

验证数据展示

  • IF Staining of C6 using CL594-60029

    IF Staining of C6 using CL594-60029

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 KMO antibody (CL594-60029, Clone: 2D2F2 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inC6 cells
For other applications, we recommend the unconjugated version of this antibody, 60029-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60029 targets KMO in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen KMO fusion protein Ag1109 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Synonyms dJ317G22.1, KMO, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase
Calculated Molecular Weight486 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC005297
Gene Symbol KMO
Gene ID (NCBI) 8564
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO15229
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KMO(Kynurenine 3-monooxygenase) is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).

实验方案

Product Specific Protocols
IF protocol for CL594 KMO antibody CL594-60029Download protocol
Standard Protocols
Click here to view our Standard Protocols