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0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 25897-1-AP (same clone as 25897-1-PBS)

    WB analysis using 25897-1-AP (same clone as 25897-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25897-1-AP (KIF5C antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

    IP experiment of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

    IP result of anti-KIF5C (IP:25897-1-AP, 4ug; Detection:25897-1-AP 1:800) with mouse brain tissue lysate 2080 ug. This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

    IHC staining of mouse brain using 25897-1-AP (same clone as 25897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25897-1-AP (KIF5C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 25897-1-AP (same clone as 25897-1-PBS)

    IF Staining of SH-SY5Y using 25897-1-AP (same clone as 25897-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KIF5C antibody (25897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 25897-1-AP (same clone as 25897-1-PBS)

    IF Staining of HeLa using 25897-1-AP (same clone as 25897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIF5C antibody (25897-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25897-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KIF5C Polyclonal antibody, PBS Only

KIF5C Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 25897-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

NKHC, KINN, Kinesin-1, Kinesin heavy chain neuron-specific 2, KIAA0531

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25897-1-PBS targets KIF5C in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KIF5C fusion protein Ag23163 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 5C
别名 NKHC, KINN, Kinesin-1, Kinesin heavy chain neuron-specific 2, KIAA0531
计算分子量 725 aa, 83 kDa
观测分子量 70 kDa, 110 kDa
GenBank蛋白编号BC110287
基因名称 KIF5C
Gene ID (NCBI) 3800
RRIDAB_2880288
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60282
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIF5C (Kinesin heavy chain isoform 5C variant) which belongs to the kinesin-like protein family is neuron specific. The kinesin-1 subfamilies (kif5a, kif5b, kif5c) are responsible for the movement of mitochondria in neurons. Kinesins have N-terminal motor domains and C-terminal cargo-binding tail domains separated by hinge regions. The hinge and C-terminal tail regions of Kif5a, Kif5b, and Kif5c bound a large detergent-resistant RNase-sensitive granule. The granule localized to dendrites and underwent bidirectional movement. Distally directed movement of the granule was enhanced by Kif5 overexpression and reduced by Kif5 functional blockage. kinesins may transport RNA in dendrites via this large granule. Two major isoforms of kif5c, with molecular predicted masses of 80 and 110 kDa, are generated by alternative splicing.