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Species Reactivity
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验证数据展示

  • WB analysis of HeLa using 13105-1-AP (same clone as 13105-1-PBS)

    WB analysis of HeLa using 13105-1-AP (same clone as 13105-1-PBS)

    WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • WB analysis using 13105-1-AP (same clone as 13105-1-PBS)

    WB analysis using 13105-1-AP (same clone as 13105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 13105-1-AP (same clone as 13105-1-PBS)

    IF Staining of A549 using 13105-1-AP (same clone as 13105-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (13105-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 13105-1-AP (same clone as 13105-1-PBS)

    IP experiment of mouse brain using 13105-1-AP (same clone as 13105-1-PBS)

    IP result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

    IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

    IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 13105-1-AP (same clone as 13105-1-PBS)

    FC experiment of A549 using 13105-1-AP (same clone as 13105-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KIF2A (13105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KIF2A Polyclonal antibody, PBS Only

KIF2A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 13105-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

HK2, KIF2, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13105-1-PBS targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, canine
免疫原 KIF2A fusion protein Ag3748 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin heavy chain member 2A
别名 HK2, KIF2, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A
计算分子量 679 aa, 77 kDa
观测分子量 80-100 kDa
GenBank蛋白编号BC031828
基因名称 KIF2A
Gene ID (NCBI) 3796
RRIDAB_2265336
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00139
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kinesin family member 2a (KIF2A), one of the kinesin family members, is identified in mammalian cells and involved in regulating microtubule dynamics during the mitosis via modulating intracellular transport, cell division, and bipolar spindle assembly (PMID: 17538014). High expression of KIF2A correlates with advanced tumor stages and worse prognosis in non-small cell lung cancer patients (PMID: 31858647). Within clinical practice, KIF2A exhibits key prognostic value for the management of gastric cancer, colorectal cancer and nasopharyngeal carcinoma (PMID: 32106376, PMID: 26070867, PMID: 26602436).