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验证数据展示

  • WB analysis using 27276-1-AP (same clone as 27276-1-PBS)

    WB analysis using 27276-1-AP (same clone as 27276-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27276-1-AP (KIF21A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 27276-1-AP (same clone as 27276-1-PBS)

    WB analysis of mouse brain using 27276-1-AP (same clone as 27276-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 27276-1-AP (KIF21A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 27276-1-AP (same clone as 27276-1-PBS)

    IP experiment of mouse brain using 27276-1-AP (same clone as 27276-1-PBS)

    IP result of anti-KIF21A (IP:27276-1-AP, 4ug; Detection:27276-1-AP 1:500) with mouse brain tissue lysate 5600ug. This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 27276-1-AP (same clone as 27276-1-PBS)

    IHC staining of human ovary cancer using 27276-1-AP (same clone as 27276-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27276-1-AP (KIF21A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27276-1-PBS in a different storage buffer formulation.

KIF21A Polyclonal antibody, PBS Only

KIF21A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27276-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

KIF2, KIAA1708, FEOM3A, FEOM1, CFEOM1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

27276-1-PBS targets KIF21A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26192

Product name: Recombinant human KIF21A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1125-1230 aa of NM_001173463

Sequence: MPLNSPGSEGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSELASDTSTGDASLPGPLTPVAEGQEIGMNTETSGTSAREKELSPPPGLPSKIGSISRQSSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 21A
别名 KIF2, KIAA1708, FEOM3A, FEOM1, CFEOM1
计算分子量 187 kDa
观测分子量 190-200 kDa
GenBank蛋白编号NM_001173463
基因名称 KIF21A
Gene ID (NCBI) 55605
RRIDAB_2880825
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z4S6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIF21A is well known for its association with congenital fibrosis of the extraocular muscles type 1 (CFEOM1). CFEOM1-related mutations in KIF21A relieve autoinhibition of the motor and third coiled-coil stalk, which results in enhanced accumulation of KIF21A in axonal growth cones and aberrant axon morphology in the oculomotor nerve.(PMID: 38767486)

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