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验证数据展示

  • WB analysis of human cerebellum using 14212-1-AP (same clone as 14212-1-PBS)

    WB analysis of human cerebellum using 14212-1-AP (same clone as 14212-1-PBS)

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14212-1-AP (KChIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 14212-1-AP (same clone as 14212-1-PBS)

    IHC staining of human pancreas cancer using 14212-1-AP (same clone as 14212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 14212-1-AP (same clone as 14212-1-PBS)

    IHC staining of human pancreas cancer using 14212-1-AP (same clone as 14212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14212-1-AP (KChIP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

  • IP experiment of fetal human brain using 14212-1-AP (same clone as 14212-1-PBS)

    IP experiment of fetal human brain using 14212-1-AP (same clone as 14212-1-PBS)

    IP result of anti-KChIP1 (IP:14212-1-AP, 4ug; Detection:14212-1-AP 1:300) with fetal human brain tissue lysate 3500 ug. This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 14212-1-AP (same clone as 14212-1-PBS)

    FC experiment of HepG2 using 14212-1-AP (same clone as 14212-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KChIP1 Polyclonal antibody (14212-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14212-1-PBS in a different storage buffer formulation.

KChIP1 Polyclonal antibody, PBS Only

KChIP1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 14212-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

KCNIP1, A-type potassium channel modulatory protein 1, Kv channel-interacting protein 1, Potassium channel-interacting protein 1, VABP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

14212-1-PBS targets KChIP1 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5432

Product name: Recombinant human KCNIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC050375

Sequence: MGAVMGTFSSLQTKQRRPSKDKIEDELEMTMVCHRPEGLEQLEAQTNFTKRELQVLYRGFKNECPSGVVNEDTFKQIYAQFFPHGDASTYAHYLFNAFDTTQTGSVKFEDFVTALSILLRGTVHEKLRWTFNLYDINKDGYINKEEMMDIVKAIYDMMGKYTYPVLKEDTPRQHVDVFFQKMDKNKDGIVTLDEFLESCQEDDNIMRSLQLFQNVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Kv channel interacting protein 1
别名 KCNIP1, A-type potassium channel modulatory protein 1, Kv channel-interacting protein 1, Potassium channel-interacting protein 1, VABP
计算分子量 227 aa, 27 kDa
观测分子量 25 kDa
GenBank蛋白编号BC050375
基因名称 KCNIP1
Gene ID (NCBI) 30820
RRIDAB_2265412
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZI2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.

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