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验证数据展示

  • WB analysis using 31900-1-AP (same clone as 31900-1-PBS)

    WB analysis using 31900-1-AP (same clone as 31900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31900-1-AP (KCNA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31900-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 31900-1-AP (same clone as 31900-1-PBS)

    IHC staining of mouse brain using 31900-1-AP (same clone as 31900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31900-1-AP (KCNA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31900-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 31900-1-AP (same clone as 31900-1-PBS)

    IHC staining of rat brain using 31900-1-AP (same clone as 31900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31900-1-AP (KCNA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31900-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 31900-1-AP (same clone as 31900-1-PBS)

    IF Staining of mouse cerebellum using 31900-1-AP (same clone as 31900-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using KCNA6 antibody (31900-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), FUS/TLS antibody (68262-1-Ig, Clone: 1B4F8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31900-1-PBS in a different storage buffer formulation.

KCNA6 Polyclonal antibody, PBS Only

KCNA6 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31900-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

HBK2, Human Brain Potassium Channel-2, Kv1.6, Potassium voltage-gated channel subfamily A member 6, PPP1R96

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

31900-1-PBS targets KCNA6 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35654

Product name: Recombinant human KCNA6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 194-262 aa of NM_002235.3

Sequence: ETLPQFRVDGRGGNNGGVSRVSPVSRGSQEEEEDEDDSYTFHHGITPGEMGTGGSSSLSTLGGSFFTDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 potassium voltage-gated channel, shaker-related subfamily, member 6
别名 HBK2, Human Brain Potassium Channel-2, Kv1.6, Potassium voltage-gated channel subfamily A member 6, PPP1R96
计算分子量59 kDa，529aa
观测分子量65 kDa
GenBank蛋白编号NM_002235.3
基因名称 KCNA6
Gene ID (NCBI) 3742
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP17658
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Shaker-like Kv1 potassium channel family comprises 8 α subunits (Kv1.1-Kv1.8), which are key regulators of neuronal excitability in mammals (PMID: 7842011; 9581771; 22612818). Potassium voltage-gated channel subfamily A member 6 (KCNA6, also known as HBK2, Kv1.6, and PPP1R96) is a multi-pass membrane protein that is mainly expressed in the central and peripheral nervous system (PMID: 12042352; 8872310). The concentration of the Kv1.6 channel in the cell membrane can modulate the kinetic properties of Kv1.6-induced K+ current (PMID: 14575698).

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