Jervine

Hedgehog:500-700 nM (IC50)

胚胎暴露于jervine时，会发展出与Shh突变胚胎中发现的类似的颅面异常。这些胚胎的下颌严重缩小，常常导致门牙缺失。用jervine处理怀孕的小鼠，会在胚胎中诱发类似于Prx1/Prx2/胚胎中发现的下颌缺陷。下颌的远端部分大小减小，两颗门牙缺失[1]。

与对照组相比，暴露于25 μg/ml jervine的软骨细胞培养显示细胞数量明显减少，但在细胞形态或alcian blue染色的相对强度上没有差异[2]。

DMSO:4.26 mg/mL (10.01 mM);Chloroform:Soluble;H2O:< 1 mg/mL (insoluble or slightly soluble)

Cells are grown in Eagle's basal medium supplemented with 10% fetal bovine serum and 0.25 mg/ml gentamicin sulfate. Cells are seeded onto 35-mm tissue culture plates at a density of 1×104 celldplate. The plated cells are incubated for 24 hours prior to treatment in a humidified incubator at 37 "C in an atmosphere of 5% CO2 in air. Drugs are then added: jervine to a final concentration of 5, 10, or 25 μg/ml; retinoic acid at 0.03, 0.30, or 3.0 pg/ml. After a final 48 hours of incubation, media are removed, the cultures are rinsed three times with phosphate-buffered saline (PBS) pH 7.4, and the cells are harvested into 0.5 ml of 0.1% (w/v) trypsin which contained 0.1% EDTA (w/v) in PBS. Each sample is mixed with 9.5 ml Isoton Ⅱ, and cell numbers are determined by using a Coulter Counter. (Only for Reference)