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验证数据展示

  • WB analysis using 25212-1-AP (same clone as 25212-1-PBS)

    WB analysis using 25212-1-AP (same clone as 25212-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25212-1-AP (JIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25212-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 25212-1-AP (same clone as 25212-1-PBS)

    IHC staining of rat brain using 25212-1-AP (same clone as 25212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25212-1-AP (JIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25212-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 25212-1-AP (same clone as 25212-1-PBS)

    IHC staining of rat brain using 25212-1-AP (same clone as 25212-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25212-1-AP (JIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25212-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

JIP3 Polyclonal antibody, PBS Only

JIP3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25212-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

MAPK8IP3, C-Jun-amino-terminal kinase-interacting protein 3, JIP 3, JIP-3, JNK interacting protein 3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25212-1-PBS targets JIP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19561

Product name: Recombinant human JIP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 171-248 aa of BC137123

Sequence: MIQTYVEHIERSKMQQVGGNSQTESSLPGRRKERPTSLNVFPLADGTVRAQIGGKLVPAGDHWHLSDLGQLQSSSSYQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 8 interacting protein 3
别名 MAPK8IP3, C-Jun-amino-terminal kinase-interacting protein 3, JIP 3, JIP-3, JNK interacting protein 3
计算分子量 1336 aa, 147 kDa
观测分子量 147 kDa
GenBank蛋白编号BC137123
基因名称 JIP3
Gene ID (NCBI) 23162
RRIDAB_2879962
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPT6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

JIP3, also known as JNK-interacting protein 3 or JSAP1 (JNK/SAPK-associated protein 1), is a crucial scaffold protein primarily involved in organizing and regulating signal transduction pathways, especially the c-Jun N-terminal kinase (JNK) pathway.

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