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验证数据展示

  • FC experiment of Transfected HEK-293T using 98594-2-RR (same clone as 98594-2-PBS)

    FC experiment of Transfected HEK-293T using 98594-2-RR (same clone as 98594-2-PBS)

    1x10^6 HEK-293T cells (left) or IL-12A/IL-12 p35 Transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat IL-12A/IL-12 p35 Rabbit RecAb (98594-2-RR, Clone:250739E10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98594-2-PBS in a different storage buffer formulation.

  • FC experiment of C6 using 98594-2-RR (same clone as 98594-2-PBS)

    FC experiment of C6 using 98594-2-RR (same clone as 98594-2-PBS)

    1x10^6 C6 cells were intracellularly stained with 0.25 ug Anti-Rat IL-12A/IL-12 p35 Rabbit RecAb (98594-2-RR, Clone: 250739E10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98594-2-PBS in a different storage buffer formulation.

  • Affinity of 98594-2-RR

    Affinity of 98594-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98594-2-RR against Rat IL-12A/IL-12 p35 were performed. The affinity constant is below 1 pM.

Anti-Rat IL-12A/IL-12 p35 Rabbit Recombinant Antibody, PBS Only

IL-12A/IL-12 p35 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98594-2-PBS

产品说明书

CloneNo. 250739E10

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

FC (Intra)

别名

CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, Il12a, IL-12A

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98594-2-PBS targets IL-12A/IL-12 p35 in FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat
免疫原

CatNo: Eg3823

Product name: Recombinant Rat IL-12A protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 23-215 aa of NM_053390.1

Sequence: RVIPVSGPAKCLNQSQNLLKTTDDMVRTAREKLKHYSCTAGDIDHEDITRDKTSTLEACLPLELHKNESCLATKETSSIIRGSCLPPQKTSLMMTLCLGSIYEDLKMYQSEFQAINAALQSHNHQQITLDRNMLMAIDELMRSLNHSGETLHQKAPMGEADPYRVKMKLCILLHAFSTRVMTINRVMNYLSSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 12a
别名 CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, Il12a, IL-12A
计算分子量24 kDa
GenBank蛋白编号NM_053390.1
基因名称 Il12a
Gene ID (NCBI) 84405
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9R103
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).

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