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验证数据展示

  • WB analysis of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

    WB analysis of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

    IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IKZF3 antibody included
  • IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

    IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IKZF3 antibody included
  • IF Staining of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

    IF Staining of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using IKZF3 antibody 19055-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

Aiolos/IKZF3 Polyclonal antibody, PBS Only

Aiolos/IKZF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 19055-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

IKZF3, ZNFN1A3, Zinc finger protein Aiolos, IKZF 3, AIOLOS

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IKZF3 Ready-To-Use IHC Kit. IKZF3 primary antibody included.

产品信息

19055-1-PBS targets Aiolos/IKZF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Aiolos/IKZF3 fusion protein Ag5537 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IKAROS family zinc finger 3 (Aiolos)
别名 IKZF3, ZNFN1A3, Zinc finger protein Aiolos, IKZF 3, AIOLOS
计算分子量 509 aa, 58 kDa
观测分子量 58-70 kDa
GenBank蛋白编号BC032707
基因名称 IKZF3
Gene ID (NCBI) 22806
RRIDAB_10643381
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKT9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation. The calculated molecular weight of IKZF3 is 58 kDa, but the modified IKZF3 protein is about 65-70 kDa.