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IFT140 Recombinant antibody

IFT140 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230231F7

Cat No : 82947-1-RR

Print datasheet

Synonyms

c305C8.4, c380F5.1, DKFZp564L232, FLJ10306, FLJ30571, gs114, IFT140, KIAA0590, WDTC2

验证数据展示

  • WB analysis of HeLa using 82947-1-RR

    WB analysis of HeLa using 82947-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 82947-1-RR (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat testis using 82947-1-RR

    WB analysis of rat testis using 82947-1-RR

    rat testis tissue were subjected to SDS PAGE followed by western blot with 82947-1-RR (IFT140 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, rat testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82947-1-RR targets IFT140 in WB, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Rat
Immunogen IFT140 fusion protein Ag11119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Synonyms c305C8.4, c380F5.1, DKFZp564L232, FLJ10306, FLJ30571, gs114, IFT140, KIAA0590, WDTC2
Calculated Molecular Weight 1462 aa, 165 kDa
Observed Molecular Weight150-165 kDa
GenBank Accession NumberBC035577
Gene Symbol IFT140
Gene ID (NCBI) 9742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96RY7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localized to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

实验方案

Product Specific Protocols
WB protocol for IFT140 antibody 82947-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols