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Recombinant Human SCF protein (Myc Tag, His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

Myc Tag, His Tag

生物活性

未测试

Cat no : Eg0112

Print datasheet

Synonyms

KITLG, c Kit ligand, c-Kit ligand, KIT ligand, Kitl

验证数据展示

  • Purity of Recombinant Human SCF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human SCF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <1.0 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Human SCF protein Glu26-Ala189 (Accession# P21583) with a Myc tag and a His tag at the C-terminus.
基因ID 4254
蛋白编号 P21583
预测分子量 24.1 kDa
SDS-PAGE
组分 Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Stem cell factor (SCF) is also named as mast cell growth factor (MGF), Kit ligand (Kitl), c-Kit ligand, steel factor and Kitlg. SCF is a pleiotropic cytokine (also as a dimeric molecule) that mediates regulatory effects on inflammation, tissue remodeling, and fibrosis through binding to and activating its receptor tyrosine kinase c-Kit. SCF is the ligand of c-Kit, a member of the RTKs family. Molecular activity of RTKs is a primary stimulus of cell motility, and expression of the SCF/c-Kit axis is associated with cell migration. SCF is a cytokine that synergizes the effects of Epo during erythropoiesis. Stem cell factor plays a well-described role in regulating the survival, proliferation, migration, and differentiation of hematopoietic progenitors, melanocytes, and germ cells. More recent studies have demonstrated SCF expression in dermal and intestinal epithelial cells, and increased SCF expression has been reported in the inflamed mucosa of individuals with IBD and colonitis.

参考文献:

1. Williams, D E et al. Cell vol. 63,1 (1990): 167-74. 
2. Lukacs, N W et al. Blood vol. 87,6 (1996): 2262-8.
3. Lennartsson, Johan, et al. Physiological reviews vol. 92,4 (2012): 1619-49.
4. Vazquez-Mellado, Maria J et al. Vitamins and hormones vol. 105 (2017): 273-296. 
5. Garcia-Hernandez, Vicky et al. Inflammatory bowel diseases vol. 29,7 (2023): 1133-1144. 
6. Carter, Eric L et al. The Journal of investigative dermatology vol. 128,7 (2008): 1861-3. 
7. Schmitt, Mark et al. Cell reports vol. 24,9 (2018): 2312-2328.e7.