Speedy™ Human EPCAM/CD326 One-Step ELISA Kit
Sensitivity
5.5 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat no : SE50113
Synonyms
EPCAM, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, CO 17A
Validation Data Gallery
Product Information
| Product name | Speedy™ Human EPCAM/CD326 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 5.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4072 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 103% | 93%-108% |
| Urine | 79% | 77%-81% |
| Cell culture supernatant | 106% | 93%-122% |
| Cell lysate | 110% | 99%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 511.8 | 8.9 | 1.7 |
| 2 | 8 | 132.4 | 2.6 | 2.0 |
| 3 | 8 | 36.7 | 1.0 | 2.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 521.2 | 13.2 | 2.5 |
| 2 | 16 | 130.8 | 3.6 | 2.8 |
| 3 | 16 | 36.6 | 0.9 | 2.5 |
Background Information
Epithelial cell adhesion molecule (EPCAM), also known as CD326, is a well-characterized type I transmembrane glycoprotein that plays a pivotal role in cell adhesion, proliferation, differentiation, and signaling. It mediates Ca2+-independent homotypic cell-cell adhesion through its extracellular domain, which is crucial for the formation and maintenance of epithelial tissues. The membrane localization of EPCAM is essential for its function in cell adhesion and signaling. Additionally, elevated levels of soluble EPCAM have been associated with tumor burden and poor prognosis in cancer patients, making it a potential biomarker for cancer diagnosis and monitoring.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EPCAM, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, CO 17A |
