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验证数据展示

  • FC experiment of human PBMCs using 98748-1-RR (same clone as 98748-1-PBS)

    FC experiment of human PBMCs using 98748-1-RR (same clone as 98748-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA class II Rabbit RecAb (98748-1-RR, Clone:253538A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98748-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98748-1-RR (same clone as 98748-1-PBS)

    FC experiment of human PBMCs using 98748-1-RR (same clone as 98748-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human HLA class II Rabbit RecAb (98748-1-RR, Clone:253538A1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (65560-1-MR, Clone: L243). Cells were then stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98748-1-PBS in a different storage buffer formulation.

Anti-Human HLA class II Rabbit Recombinant Antibody, PBS Only

HLA class II Uni-rAb® Recombinant Antibody for FC
Cat No. 98748-1-PBS

产品说明书

CloneNo. 253538A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

MHC II, HLA-D, MHC class II

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98748-1-PBS targets HLA class II in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

unknow

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HLA class II
别名 MHC II, HLA-D, MHC class II
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system (PMID: 667938; 3375250). This antibody can recognize HLA-DR, -DP, and -DQ.

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