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验证数据展示

  • IF Staining of HeLa using 10066-2-AP (same clone as 10066-2-PBS)

    IF Staining of HeLa using 10066-2-AP (same clone as 10066-2-PBS)

    Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • WB analysis of human liver using 10066-2-AP (same clone as 10066-2-PBS)

    WB analysis of human liver using 10066-2-AP (same clone as 10066-2-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

    WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

    WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

    IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

    IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

    IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

    IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HINFP Polyclonal antibody, PBS Only

HINFP Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 10066-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

10066-2-PBS targets HINFP in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0115

Product name: Recombinant human HINFP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 215-452 aa of BC001073

Sequence: LDHIRRQTSLDQQHFQCSHCSKRFATERLLRDHMRNHVNHYKCPLCDMTCPLPSSLRNHMRFRHSEDRPFKCDCCDYSCKNLIDLQKHLDTHSEEPAYRCDFENCTFSARSLCSIKSHYRKVHEGDSEPRYKCHVCDKCFTRGNNLTVHLRKKHQFKWPSGHPRFRYKEHEDGYMRLQLVRYESVELTQQLLRQPQEGSGLGTSLNESSLQGIILETVPGEPGRKEEEEEGKGSEGTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histone H4 transcription factor
别名
计算分子量 60 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC001073
基因名称 HINFP
Gene ID (NCBI) 25988
RRIDAB_2117412
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQA5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.

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