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验证数据展示

  • WB analysis using 67818-1-Ig (same clone as 67818-1-PBS)

    WB analysis using 67818-1-Ig (same clone as 67818-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67818-1-Ig (HGS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HGS antibody included
  • IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    IHC staining of mouse brain using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67818-1-Ig (HGS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HGS antibody included
  • IF Staining of HepG2 using 67818-1-Ig (same clone as 67818-1-PBS)

    IF Staining of HepG2 using 67818-1-Ig (same clone as 67818-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HGS antibody (67818-1-Ig, Clone: 3B10D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67818-1-PBS in a different storage buffer formulation.

HGS Monoclonal antibody, PBS Only

HGS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67818-1-PBS

产品说明书

CloneNo. 3B10D6

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, ELISA

别名

HGS, HRS, Protein pp110, ZFYVE8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HGS Ready-To-Use IHC Kit. HGS primary antibody included.

产品信息

67818-1-PBS targets HGS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag28610

Product name: Recombinant human HGS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 186-387 aa of BC003565

Sequence: GQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQLNRKAEGKATSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERLRQKSTYTSYPKAEPMPSASSAPPASSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSAPVPLTEPAAQPGEGHAAPTNVVENPLPETDSQPIPPS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 hepatocyte growth factor-regulated tyrosine kinase substrate
别名 HGS, HRS, Protein pp110, ZFYVE8
计算分子量 86 kDa
观测分子量110 kDa
GenBank蛋白编号BC003565
基因名称 HGS
Gene ID (NCBI) 9146
RRIDAB_2918581
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO14964
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Hepatocyte growth factor-regulated tyrosine kinase substrate (HGS, synonyms: HRS, ZFYVE8) is a 110 to 115-kDa zinc finger phosphotyrosine protein inducible by stimulation with interleukin 2 (IL-2), granulocyte-macrophage colony-stimulating factor (GM-CSF) as well as hepatocyte growth factor (HGF), and is associated with signal-transducing adaptor molecule (STAM). HGS suppresses DNA synthesis upon stimulation with IL-2 and GM-CSF, counteracting the function of STAM which is critical for cell growth signaling mediated by the cytokines. HGS also interacts with the neurofibromatosis 2 tumor suppressor protein schwannomin/merlin. The growth suppression activity of schwannomin/merlin requires HGS and the binding of schwannomin/merlin to HGS facilitates its ability to function as a tumor suppressor, probably by inhibiting STAT activation.

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