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验证数据展示

  • WB analysis of K-562 using 11955-1-AP (same clone as 11955-1-PBS)

    WB analysis of K-562 using 11955-1-AP (same clone as 11955-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

    IP experiment of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

    IP result of anti-HELLS (IP:11955-1-AP, 4ug; Detection:11955-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)

    IHC staining of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)

    IHC staining of mouse thymus using 11955-1-AP (same clone as 11955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

    IHC staining of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

    IHC staining of mouse testis using 11955-1-AP (same clone as 11955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 11955-1-AP (same clone as 11955-1-PBS)

    IHC staining of rat testis using 11955-1-AP (same clone as 11955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 11955-1-AP (same clone as 11955-1-PBS)

    IHC staining of rat testis using 11955-1-AP (same clone as 11955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IHC staining of rat thymus using 11955-1-AP (same clone as 11955-1-PBS)

    IHC staining of rat thymus using 11955-1-AP (same clone as 11955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IHC staining of rat thymus using 11955-1-AP (same clone as 11955-1-PBS)

    IHC staining of rat thymus using 11955-1-AP (same clone as 11955-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11955-1-AP (same clone as 11955-1-PBS)

    IF Staining of HepG2 using 11955-1-AP (same clone as 11955-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HELLS antibody (11955-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 11955-1-AP (same clone as 11955-1-PBS)

    FC experiment of HepG2 using 11955-1-AP (same clone as 11955-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HELLS Polyclonal antibody (11955-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 11955-1-PBS in a different storage buffer formulation.

HELLS Polyclonal antibody, PBS Only

HELLS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 11955-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

11955-1-PBS targets HELLS in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2558

Product name: Recombinant human HELLS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-348 aa of BC031004

Sequence: MFGSSEKETIELSPTGRPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKLQNIMMLLRKCCNHPYLIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFNFSRLDGSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 helicase, lymphoid-specific
别名 Nbla10143, Lymphoid-specific helicase, Lymphoid specific helicase, LSH, helicase, lymphoid
计算分子量 348 aa, 40 kDa, 97 kDa
观测分子量 97 kDa
GenBank蛋白编号BC031004
基因名称 HELLS
Gene ID (NCBI) 3070
RRIDAB_2117529
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRZ9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.

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