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CoraLite® Plus 488-conjugated HDAC9 Monoclonal antibody

HDAC9 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G6C5

Cat No : CL488-67364

Print datasheet

Synonyms

DKFZp779K1053, HD7, HD7b, HD9, HDAC, HDAC7, HDAC7B, HDAC9, HDAC9B, HDAC9FL, HDRP, Histone deacetylase 7B, histone deacetylase 9, KIAA0744, MITR

验证数据展示

  • FC experiment of HeLa using CL488-67364

    FC experiment of HeLa using CL488-67364

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HDAC9 (CL488-67364, Clone:1G6C5) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67364-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67364 targets HDAC9 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen HDAC9 fusion protein Ag28514 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name histone deacetylase 9
Synonyms DKFZp779K1053, HD7, HD7b, HD9, HDAC, HDAC7, HDAC7B, HDAC9, HDAC9B, HDAC9FL, HDRP, Histone deacetylase 7B, histone deacetylase 9, KIAA0744, MITR
Calculated Molecular Weight 1011 aa, 111 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC152405
Gene Symbol HDAC9
Gene ID (NCBI) 9734
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKV0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HDAC9, also named as Histone deacetylase 7B, is a 1011 amino acid protein, which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC9 represses MEF2-dependent transcription. HDAC9 is broadly expressed, with highest levels in brain, heart, muscle and testis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 HDAC9 antibody CL488-67364Download protocol
Standard Protocols
Click here to view our Standard Protocols