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GSTM2 Polyclonal antibody

GSTM2 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 55474-1-AP

Print datasheet

Synonyms

Glutathione S transferase Mu 2, GST class mu 2, GST4, GSTM, GSTM2, GSTM2 2, GTHMUS

验证数据展示

  • WB analysis using 55474-1-AP

    WB analysis using 55474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55474-1-AP (GSTM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 55474-1-AP

    WB analysis using 55474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55474-1-AP (GSTM2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 55474-1-AP

    IF Staining of HeLa using 55474-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM2 antibody (55474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55474-1-AP targets GSTM2 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutathione S-transferase mu 2 (muscle)
Synonyms Glutathione S transferase Mu 2, GST class mu 2, GST4, GSTM, GSTM2, GSTM2 2, GTHMUS
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberNM_000848
Gene Symbol GSTM2
Gene ID (NCBI) 2946
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28161
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutathione S-Transferase Mu 2 (GSTM2) is a phase II metabolizing enzyme that conjugates glutathione to electrophilic compounds. GSTM2 is closely related to cancer progression, and there have been several publications regarding the function of GSTM2 in drug resistance (PMID: 36038661). Western blot analysis detected GSTM2 at an apparent molecular mass of 26 kDa.

实验方案

Product Specific Protocols
WB protocol for GSTM2 antibody 55474-1-APDownload protocol
IF protocol for GSTM2 antibody 55474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols