验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87659-1-RR targets GGT1 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse, rat |
| 免疫原 |
CatNo: Eg6301 Product name: Recombinant mouse CD224/GGT1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 27-568 aa of NM_001305992.1 Sequence: PYTSGKPDHVYSRAAVATDAKRCSEIGRDILQEGGSVVDAAIASLLCMGLMNAHSMGIGGGLFFTIYNSTTGKVEVINAREVAPRLANTTMFNNSKDSEEGGLSVAVPGEIRGYELAHQRHGRLPWARLFQPSIQLARHGFPVGKGLAIALDKKRDVIEKTPALCEVFCRQGKVLQEGETVTMPKLADTLQILAQEGAKAFYNGSLTAQIVKDIQEAGGIMTVEDLNNYRAELIEHPMSIGLGDATLYVPSAPLSGPVLILILNILKGYNFSPKSVATPEQKALTYHRIVEAFRFAYAKRTMLGDPKFVDVSQVIRNMSSEFYATQLRARITDETTHPAAYYEPEFYLQDDGGTAHLSAVSEDGSAVAATSTINLYFGSKVLSRVSGILFNDEMDDFSSPNFINQFRVAPSPANFIKPGKQPLSSMCPSIILDKDGQVRMVVGASGGTQITTSVALAIINSLWFGYDVKRAVEEPRLHNQLLPNTTTVEKDIDQVVTAGLKIRHHHTEVTPTFIAVVQAVVRASGGWAAASDSRKGGEPAGY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | gamma-glutamyltransferase 1 |
| 别名 | CD224, EC:2.3.2.2, EC:3.4.19.13, EC:3.4.19.14, Gamma-glutamyltransferase 1 |
| 计算分子量 | 62 kDa |
| 观测分子量 | 50-61 kDa |
| GenBank蛋白编号 | NM_001305992.1 |
| 基因名称 | Ggt1 |
| Gene ID (NCBI) | 14598 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q60928 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GGT1 is a heterodimer composed of light chain and heavy chain, and its active site is located in the light chain. The metabolism of glutathione by GGT1 releases free glutamic acid and dipeptide cysteine-glycine, which can be hydrolyzed into cysteine and glycine by dipeptidase. In the presence of high concentration of dipeptide and some amino acids, it can also catalyze the peptide transfer reaction and transfer γ -glutamyl group to receptor amino acids to form new γ -glutamyl compounds. Participate in cysteine homeostasis, glutathione homeostasis and the transformation of leukotriene LTC4 to LTD4 (PMID:12810527).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GGT1 antibody 87659-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
