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验证数据展示

  • WB analysis using 30601-1-AP (same clone as 30601-1-PBS)

    WB analysis using 30601-1-AP (same clone as 30601-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30601-1-PBS in a different storage buffer formulation.

  • WB analysis using 30601-1-AP (same clone as 30601-1-PBS)

    WB analysis using 30601-1-AP (same clone as 30601-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30601-1-AP (Fas/CD95 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30601-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 30601-1-AP (same clone as 30601-1-PBS)

    IF Staining of Raji using 30601-1-AP (same clone as 30601-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (30601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30601-1-PBS in a different storage buffer formulation.

Mouse Fas/CD95 Polyclonal antibody, PBS Only

Fas/CD95 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30601-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

30601-1-PBS targets Fas/CD95 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30426

Product name: Recombinant mouse Fas/CD95 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-169 aa of NM_007987

Sequence: QGTNSISESLKLRRRVRETDKNCSEGLYQGGPFCCQPCQPGKKKVEDCKMNGGTPTCAPCTEGKEYMDKNHYADKCRRCTLCDEEHGLEVETNCTLTQNTKCKCKPDFYCDSPGCEHCVRCASCEHGTLEPCTATSNTNCRKQSPRNR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fas (TNF receptor superfamily member 6)
别名
计算分子量 37 kDa
观测分子量 30-40 kDa
GenBank蛋白编号NM_007987
基因名称 Fas/CD95
Gene ID (NCBI) 14102
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25446
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAS, also named as CD95, APO-1, APT1, FAS1 and TNFRSF6, is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily, can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.

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