• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 13003-2-AP (same clone as 13003-2-PBS)

    WB analysis using 13003-2-AP (same clone as 13003-2-PBS)

    TNF alpha and IFN gamma treated HepG2 cells were subjected to SDS PAGE followed by western blot with 13003-2-AP (FAT10 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 13003-2-AP (same clone as 13003-2-PBS)

    IHC staining of human tonsillitis using 13003-2-AP (same clone as 13003-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBD antibody included
  • IHC staining of human stomach cancer using 13003-2-AP (same clone as 13003-2-PBS)

    IHC staining of human stomach cancer using 13003-2-AP (same clone as 13003-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBD antibody included
  • IHC staining of human lymphoma using 13003-2-AP (same clone as 13003-2-PBS)

    IHC staining of human lymphoma using 13003-2-AP (same clone as 13003-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBD antibody included
  • IHC staining of human lung cancer using 13003-2-AP (same clone as 13003-2-PBS)

    IHC staining of human lung cancer using 13003-2-AP (same clone as 13003-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13003-2-AP (FAT10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13003-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBD antibody included
  • Featured Product
  • KD/KO验证

FAT10 Polyclonal antibody, PBS Only

FAT10 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13003-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy UBD Ready-To-Use IHC Kit. UBD primary antibody included.

产品信息

13003-2-PBS targets FAT10 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3680

Product name: Recombinant human UBD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-165 aa of BC012472

Sequence: MAPNASCLCVHVRSEEWDLMTFDANPYDSVKKIKEHVRSKTKVPVQDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFLVESGDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTCNGKRLEDGKMMADYGIRKGNLLFLACYCIGG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin D
别名
计算分子量 165 aa, 18 kDa
观测分子量20 kDa, 70kDa
GenBank蛋白编号BC012472
基因名称 FAT10
Gene ID (NCBI) 10537
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15205
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAT10, also named UBD, contains two ubiquitin-like domains. It is a ubiquitin-like protein modifier that can be covalently attached to the target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. FAT10 also has important roles in cell mitosis, chromosome instability, apoptosis, and immune response. FAT10 mediates apoptosis in a caspase-dependent manner, especially in the renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). It promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). FAT10 regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. It may be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. FAT10 may be a marker for precancerous lesions and may promote cancer progression. This antibody is a rabbit polyclonal antibody raised against full-length FAT10 of human origin.