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验证数据展示

  • WB analysis of siRNA cells using 21529-1-AP (same clone as 21529-1-PBS)

    WB analysis of siRNA cells using 21529-1-AP (same clone as 21529-1-PBS)

    WB result of FAM120A antibody (21529-1-AP) with siRNA control and siRNA-FAM120A. This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 21529-1-AP (same clone as 21529-1-PBS)

    WB analysis of HEK-293 using 21529-1-AP (same clone as 21529-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21529-1-AP (FAM120A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 21529-1-AP (same clone as 21529-1-PBS)

    IHC staining of human stomach cancer using 21529-1-AP (same clone as 21529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21529-1-AP (FAM120A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 21529-1-AP (same clone as 21529-1-PBS)

    IF Staining of HepG2 using 21529-1-AP (same clone as 21529-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM120A antibody (21529-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

FAM120A Polyclonal antibody, PBS Only

FAM120A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21529-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C9orf10, Constitutive coactivator of PPAR-gamma-like protein 1, KIAA0183, OSSA, Oxidative stress-associated SRC activator

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

21529-1-PBS targets FAM120A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16149

Product name: Recombinant human FAM120A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 285-538 aa of BC111736

Sequence: AVADYVRNIQDTSDLDAIAKDVFQHSQSRTDDKVIRFKRAIGYYSATSKPMSFHPPHYLAARPGPFGMPGMVPPHVPPQMLNIPQTSLQAKPVAPQVPSPGGAPGQGPYPYSLSEPAPLTLDTSGKNLTEQNSYSNIPHEGKHTPLYERSSPINPAQSGSPNHVDSAYFPGSSTSSSSDNDEGSGGATNHISGNKIGWEKTGSHSEPQARGDPGDQTKAEGSSTASSGSQLAEGKGSQMGTVQPIPCLLSMPTR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 120A
别名 C9orf10, Constitutive coactivator of PPAR-gamma-like protein 1, KIAA0183, OSSA, Oxidative stress-associated SRC activator
计算分子量 1118 aa, 122 kDa
观测分子量 125 kDa
GenBank蛋白编号BC111736
基因名称 FAM120A
Gene ID (NCBI) 23196
RRIDAB_10860261
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZB2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAM120A (also named OSSA/C9ORF10) is mapped to chromosome 9q22.31, which was first reported to be a component of Purα-containing mRNP complexes. FAM120A as a transcription co-activator that couples transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1-serine/arginine-rich protein kinase 2 (SRPK2) signaling. FAM120A is abundantly expressed in the cytoplasm of the GC cells and elevated FAM120A is observed in the scirrhous-type GC tissues compared with normal gastric mucosa. (PMID: 38565940, PMID: 37595559)

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