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验证数据展示

  • IF Staining of HeLa using 55441-1-AP (same clone as 55441-1-PBS)

    IF Staining of HeLa using 55441-1-AP (same clone as 55441-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ESR2 Beta 3 antibody (55441-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red) DAPI (blue). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervix using 55441-1-AP (same clone as 55441-1-PBS)

    IHC staining of human cervix using 55441-1-AP (same clone as 55441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervix using 55441-1-AP (ESR2 Beta-3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55441-1-PBS in a different storage buffer formulation.

ESR2 Beta 3 Polyclonal antibody, PBS Only

ESR2 Beta 3 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 55441-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

55441-1-PBS targets ESR2 Beta 3 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 estrogen receptor 2 (ER beta)
别名
计算分子量 58 kDa
GenBank蛋白编号NM_001437
基因名称 ESR2
Gene ID (NCBI) 2100
RRIDAB_2881334
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92731
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual