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验证数据展示

  • WB analysis using 31432-1-AP (same clone as 31432-1-PBS)

    WB analysis using 31432-1-AP (same clone as 31432-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31432-1-AP (ERMN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31432-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 31432-1-AP (same clone as 31432-1-PBS)

    IF Staining of mouse brain using 31432-1-AP (same clone as 31432-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31432-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 31432-1-AP (same clone as 31432-1-PBS)

    IF Staining of rat brain using 31432-1-AP (same clone as 31432-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31432-1-PBS in a different storage buffer formulation.

ERMN Polyclonal antibody, PBS Only

ERMN Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 31432-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, Indirect ELISA

别名

KIAA1189, Juxtanodin, JN, Ermin

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

31432-1-PBS targets ERMN in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35648

Product name: Recombinant human ERMN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-257 aa of BC026345

Sequence: MTDVPATFTQAECNGDKPPENGQQTITKISEELTDVDSPLPHYRVEPSLEGALTKGSQEERRKLQGNMLLNSSMEDKMLKENPEEKLFIVHKAITDLSLQETSADEMTFREGHQWEKIPLSGSNQEIRRQKERITEQPLKEEEDEDRKNKGHQAAEIEWLGFRKPSQADMLHSKHDEEQKVWDEEIDDDDDDNCNNDEDEVRVIEFKKKHEEVSQFKEEGDASEDSPLSSASSQAVTPDEQPTLGKKSDISRNAYSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ermin, ERM-like protein
别名 KIAA1189, Juxtanodin, JN, Ermin
观测分子量 45 kDa
GenBank蛋白编号BC026345
基因名称 ERMN
Gene ID (NCBI) 57471
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8TAM6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERMN is associated with cytoskeletal rearrangements and the stability of myelin sheath. A study has shown that ERMN is expressed specifically in oligodendrocytes in the central nervous system (CNS), and is involved in the formation of myelin (PMID: 20934411). The predicted MW of ERMN is 33 KDa, while western blot analyses detected a 45-50 kDa single band protein as a result of phosphorylation modification.

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