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验证数据展示

  • WB analysis using 31301-1-AP (same clone as 31301-1-PBS)

    WB analysis using 31301-1-AP (same clone as 31301-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31301-1-AP (EPS15 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31301-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 31301-1-AP (same clone as 31301-1-PBS)

    WB analysis of MCF-7 using 31301-1-AP (same clone as 31301-1-PBS)

    WB result of EPS15 antibody (31301-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS15 transfected MCF-7 cells. This data was developed using the same antibody clone with 31301-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EPS15 Polyclonal antibody, PBS Only

EPS15 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 31301-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

AF 1P, AF1P, MLLT5, Protein AF 1p, Protein Eps15

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EPS15 Ready-To-Use IHC Kit. EPS15 primary antibody included.

产品信息

31301-1-PBS targets EPS15 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35282

Product name: Recombinant human EPS15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 700-860 aa of NM_001981.3

Sequence: VVAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epidermal growth factor receptor pathway substrate 15
别名 AF 1P, AF1P, MLLT5, Protein AF 1p, Protein Eps15
计算分子量99 kDa
观测分子量140 kDa
GenBank蛋白编号NM_001981.3
基因名称 EPS15
Gene ID (NCBI) 2060
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP42566
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPS15 was originally identified as a substrate for the kinase activity of the epidermal growth factor receptor (EGFR). EPS15 has a tripartite structure comprising an NH2-terminal portion, which contains three EH domains, a central putative coiled-coil region, and a COOH-terminal domain containing multiple copies of the amino acid triplet Aspartate-Proline-Phenylalanine (PMID: 10481267). EPS15 is phosphorylated on tyrosine residues following EGFR activation (PMID: 7689153).

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